Table 3.
Ingredients (g/kg diet) and analyzed proximate composition (%) of the experimental diets supplemented with different levels of vitamin D3 and vitamin K3.
| Experimental diet | |||||||
|---|---|---|---|---|---|---|---|
| Diet | 0 | 0.06/70 | 0.06/170 | 0.13/70 | 0.13/170 | 0.40/70 | 0.40/170 |
| Ingredients (%) | |||||||
| Squid powder∗ | 70.2 | 70.2 | 70.2 | 70.2 | 70.2 | 70.2 | 70.2 |
| Gelatin | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
| Krill oil† | 13 | 13 | 13 | 13 | 13 | 13 | 13 |
| Mineral premix˟ | 4.5 | 4.5 | 4.5 | 4.5 | 4.5 | 4.5 | 4.5 |
| Sel-Plex | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 |
| Vitamin premix˟ | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
| Attractantsᵜ | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
| Proximate analysis (% wet weight) | |||||||
| Moisture | 8.2 ± 0.16 | 6.79 ± 0.01 | 7.47 ± 0.04 | 7.47 ± 0.16 | 7.99 ± 0.09 | 8.9 ± 0.04 | 7.86 ± 0.03 |
| Ash | 7.44 ± 0.03 | 7.59 ± 0.02 | 7.51 ± 0.02 | 7.49 ± 0.04 | 7.48 ± 0.02 | 7.39 ± 0.01 | 7.07 ± 0.49 |
| Lipid | 22.03 ± 0.96 | 20.36 ± 0.33 | 20.09 ± 0.38 | 20.25 ± 0.01 | 20.63 ± 0.58 | 19.46 ± 0.54 | 19.83 ± 0.83 |
| Protein | 63.72 ± 0.76 | 65.33 ± 0.28 | 63.9 ± 1.85 | 65.46 ± 0.52 | 64.71 ± 0.4 | 64.88 ± 0.02 | 65.34 ± 0.06 |
∗Bacarel Express, code: 70400, United Kingdom. †Krill, high phospholipids, Aker BioMarine, Fjordalléen, Norway. ˟Vitamin and mineral premix used according to [86] with modifications: vitamin premix (mg/100 g): water-soluble vitamins (cyanocobalamin: 0.03, astaxanthin: 5, folic acid: 5.44, pyridoxine-HCl: 17.28, thiamine-HCl: 21.77, riboflavin: 72.53, calcium pantothenate: 101.59, P-aminobenzoic acid: 145, nicotinic acid: 290.16, and inositol: 1450.9) and fat-soluble vitamins (retinol acetate: 0.24, alpha-tocopherol acetate: 150, and vitamin C: 180). Mineral premix (mg/100 g): sodium chloride (NaCl): 215.133, magnesium sulfate heptahydrate (MgSO4.7H2O): 677.545, sodium dihydrogen phosphate monohydrate (NaH2PO4.H2O): 381.453, dipotassium hydrogen phosphate (K2HPO4): 758.949, calcium dihydrogen phosphate dihydrate (Ca(H2PO4).2H2O): 671.61, ferric citrate (FeC6H5O7): 146.884, calcium lactate (C3H5O3.1/2Ca): 1617.21, aluminum sulfate hexahydrate (Al2(SO4)3.6H2O): 0.693, zinc sulfate heptahydrate (ZnSO4.7H2O): 14.837, copper sulfate pentahydrate (CuSO4.5H2O): 1.247, manganese sulfate monohydrate (MnSO4.H2O): 2.998, potassium iodide (KI): 0.742, and cobalt sulfate heptahydrate (CoSO4.7H2O): 10.706. ᵜAttractants were used based on [87].