Table 4.
Sequences of primers used in gene expression studies.
| Gene | Nucleotide sequence (5′-3′) | Annealing temperature | Accession number |
|---|---|---|---|
| Beta-actin (β-actin) | F: TCTGTCTGGATCGGAGGCTC | 58.1 | X89920 |
| R: AAGCATTTGCGGTGGACG | |||
| Elongation factor 1-alpha (ef1α) | F: CTTCAACGCTCAGGTCATCAT | 60 | AF184170 |
| R: GCACAGCGAAACGACCAAGGGGA | |||
| Ribosomal protein L27 (rpl27) | F: AAGAGGAACACAACTCACTGCCCCAC | 68 | AY188520 |
| R: GCTTGCCTTTGCCCAGAACTTTGTAG | |||
| Bone morphogenic protein 2 (bmp2) | F: GTGGCTTCCATCGTATCAACATTTT | 60 | JF261172.1 |
| R: GCTCCCCGCCATGAGT | |||
| RUNX family transcription factor 2 (runx2) | F: GCCTGTCGCCTTTAAGGTGGTTGC | 61 | AJ619023 |
| R: TCGTCGTTGCCCGCCATAGCTG | |||
| Osterix (osx) | F: CAGTCAGGGATTCAGCAACA | 60 | ERR22591_isotig06993 |
| R: GGTGAAGGAGCCAGTGTAGG | |||
| Alkaline phosphatase (alp) | F: AGAACGCCCTGACGCTGCAA | 61 | AY266359 |
| R: TTCAGTATACGAGCAGCCGTCAC | |||
| Osteocalcin (oc) | F: GGCAGCCATCTGTCTGACTT | 58.1 | AF048703 |
| R: GGTCCGTAGTAGGCCGTGTA | |||
| Matrix Gla protein (mgp) | F: CGCCCGAAATACACCTCAGA | 60 | AY065652 |
| R: GACGGACGGATACTAGGAGTCTA | |||
| Calcium-sensing receptor (casr) | F: GCTTCTCCAGCTCGCTCATC | 60 | AJ289717 |
| R: AGGCGGGCTGGCGTAA | |||
| Parathyroid hormone 1 receptor (pthr1) | F: GAACCTGCCCGGCTACGTGAAG | 60 | AJ619024 |
| R: GCTCCTGTCCCG ACGAGGGTAT | |||
| Parathyroid hormone-related protein (pthrp) | F: GAGGCAAATGAATGGAACAG | 60 | AF197904 |
| R: TGGCCAGCTCAAAACTTGT |