. 2023 May 23;2023:3061649. doi: 10.1155/2023/3061649

Table 6.

Relative gene expression of bone biomarkers and calcium regulators in gilthead seabream larvae fed diets supplemented with several levels of vitamin D₃ and vitamin K₃ for 21 days.

	Diet								One-way ANOVA (p value)	Two-way ANOVA (p value)
	Gene	0	0.06/70	0.06/170	0.13/70	0.13/170	0.40/70	0.40/170	One-way ANOVA (p value)	VD	VK	VD^∗VK
Day 7 (29 dph)	bmp2	1.08 ± 0.40	1.13 ± 1.11	1.09 ± 0.62	0.96 ± 0.64	0.42 ± 0.15	1.11 ± 0.79	0.79 ± 0.33	0.659	0.751	0.267	0.48
	runx2	1.04 ± 0.31	0.77 ± 0.44	1.27 ± 0.53	1.16 ± 0.72	1.04 ± 0.55	1.14 ± 0.56	1.12 ± 0.56	0.892	0.473	0.592	0.185
	osx	1.06 ± 0.41	0.83 ± 0.14	1.24 ± 0.71	0.78 ± 0.21	0.80 ± 0.45	0.67 ± 0.08	1.53 ± 1.19	0.201	0.189	0.859	0.164
	alp	1.00 ± 0.10	1.15 ± 0.07	1.74 ± 0.71	1.34 ± 0.36	1.09 ± 0.22	1.16 ± 0.51	1.31 ± 0.44	0.102	0.674	0.459	0.371
	oc	1.05 ± 0.32^b	0.92 ± 0.57^b	1.15 ± 0.47^b	0.70 ± 0.48^ab	0.29 ± 0.07^a	0.52 ± 0.15^ab	1.14 ± 0.72^b	0.03	0.055	0.547	0.344
	mgp	1.03 ± 0.24^ab	1.01 ± 0.28^ab	1.07 ± 0.32^ab	0.87 ± 0.31^ab	0.44 ± 0.12^a	0.68 ± 0.27^ab	1.31 ± 0.57^b	0.009	0.056	0.297	0.067
	casr	1.16 ± 0.66^ab	0.88 ± 0.32^ab	0.92 ± 0.60^ab	1.03 ± 0.65^ab	0.35 ± 0.06^a	0.76 ± 0.14^ab	1.90 ± 1.30^b	0.054	0.104	0.501	0.015
	pthr1	1.04 ± 0.29^ab	0.88 ± 0.45^ab	0.82 ± 0.49^ab	0.76 ± 0.38^ab	0.34 ± 0.02^a	0.55 ± 0.16^ab	1.15 ± 0.53^b	0.035	0.488	0.457	0.113
	pthrp	1.09 ± 0.45^ab	1.04 ± 0.69^ab	0.99 ± 0.51^ab	1.10 ± 0.84^ab	0.35 ± 0.05^a	0.80 ± 0.23^ab	1.97 ± 0.95^b	0.035	0.068	0.595	0.005

Day 21 (43 dph)	bmp2	1.03 ± 0.29^ab	1.19 ± 0.47^b	0.70 ± 0.30^ab	0.67 ± 0.31^ab	0.75 ± 0.33^ab	0.64 ± 0.26^ab	0.55 ± 0.26^a	0.026	0.178	0.352	0.51
	runx2	1.02 ± 0.25	0.93 ± 0.36	0.80 ± 0.43	1.02 ± 0.31	1.02 ± 0.70	1.35 ± 0.90	0.95 ± 0.29	0.705	0.746	0.28	0.519
	osx	1.01 ± 0.17^ab	1.20 ± 0.23^b	0.67 ± 0.19^a	0.67 ± 0.22^a	0.75 ± 0.19^ab	0.66 ± 0.29^a	1.12 ± 0.46^ab	0.002	0.122	0.185	0.043
	alp	1.03 ± 0.26	1.12 ± 0.33	1.05 ± 0.38	1.37 ± 0.47	1.20 ± 0.24	1.77 ± 0.81	1.68 ± 0.55	0.054	0.141	0.555	0.529
	oc	1.06 ± 0.43	1.71 ± 0.46	0.98 ± 0.61	1.50 ± 0.82	1.04 ± 0.60	0.78 ± 0.50	0.79 ± 0.51	0.149	0.058	0.002	0.284
	mgp	1.03 ± 0.29^abc	1.33 ± 0.50^c	0.81 ± 0.24^ab	0.83 ± 0.27^ab	0.85 ± 0.19^ab	0.74 ± 0.18^a	1.22 ± 0.54^bc	0.037	0.054	0.059	0.039
	casr	1.03 ± 0.27	1.21 ± 0.75	0.70 ± 0.29	1.20 ± 0.53	0.98 ± 0.21	0.99 ± 0.23	1.40 ± 0.48	0.215	0.667	0.662	0.175
	pthr1	1.00 ± 0.12^b	0.84 ± 0.25^ab	0.49 ± 0.26^a	0.63 ± 0.21^ab	0.48 ± 0.12^a	0.64 ± 0.38^ab	0.51 ± 0.25^a	0.014	0.694	0.103	0.691
	pthrp	1.02 ± 0.19^ab	1.64 ± 0.50^b	0.78 ± 0.20^a	1.61 ± 0.31^b	1.00 ± 0.27^ab	1.13 ± 0.58^ab	1.18 ± 0.23^ab	0.002	0.531	0.002	0.053

^∗Different letters in a row denote significant differences between groups fed different diets (mean ± SD, n = 3, p < 0.05).