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. 2023 Feb 9;19(6):731–739. doi: 10.1038/s41589-023-01265-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a-b, Comparison between Antares-CFz and FLuc-D-luciferin/CycLuc1 in Vglut2-driven luciferase-expressing reporter mice. a, Representative images with peak bioluminescence from each substrate injection. b, Bioluminescence intensity in the brain over time. Data are presented as mean values ± SEM. n = 3 animals in each group. c–e, Comparison between Antares-CFz/FFz and AkaLuc-AkaLumine pairs in the hippocampus of J:NU mice co-infected with AAV expressing Antares and AAV expressing AkaLuc. c, Representative images with peak bioluminescence from each substrate injection. d, Mean peak signal intensities. Data are presented as mean values ± SEM. P = 0.0457 between Antares-CFz and AkaLuc-AkaLumine and P = 0.0062 between Antares-CFz and Antares-FFz by two-tailed Student’s paired t-test. n = 4 animals in each group.