Extended Data Fig. 10. Measurements of the ultrasound-induced thermal effect in vitro.

(A) Temperature changes in the cell culture chamber (ΔT_chamber) as a function of time. (B) (Left) The activation onset time of TRPM2⁺ cells was calculated from the fluorescence Ca²⁺ images of in vitro HEK293 cells with TRPM2 overexpression (Fig. 6). (Right) The corresponding ΔT_chamber at the onset time of TRPM2 activation. Solid lines and shadows denote the mean ± s.e.m. Each dot represents one cell.

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