Extended Data Fig. 3. Effect of the amount of 7D12-109Bpa on the photocrosslinking efficiency between 7D12-109Bpa and EGFR assessed using denaturing SDS–PAGE.
For these experiments, the amount of EGFR is 10 picomoles, incubation time prior to irradiation is 5 min, irradiation time with 365 nm light is 10 min, and the amount of 7D12-109Bpa is varied from 0 to 200 picomoles. (A) In the Coomassie stained gel image, the band higher than EGFR is photocrosslinked 7D12-109Bpa + EGFR complex. This is the full gel image for data shown in Fig. 2d. Lane marked L is the Invitrogen SeeBlue Plus2 Pre-stained Protein Standard (Catalog no. LC5925). These results demonstrate that the photocrosslinking efficiency plateaus at 100 picomoles of 7D12-109Bpa. (B) Percentage crosslinked product at different amounts of 7D12-109Bpa calculated by measuring the intensity of the band corresponding to 7D12-109Bpa + EGFR and EGFR.