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. 2023 May 30;13:8773. doi: 10.1038/s41598-023-35511-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Localization of the newly identified PTP7 at the terminal end of the extruded polar tube. (a) IFA with anti-PTP7 mouse antiserum (dilution 1:100) revealing a specific labelling of the terminal end of the A. algerae extruded polar tube (arrow). Secondary antibodies were Alexa 488-conjugated goat anti-mouse IgG. At least 10 different fields were observed which corresponds to ~ 50 to 130 spores with their polar tube extruded. See Supplementary Fig. S2 for additional pictures illustrating the different localizations of PTP7. PT polar tube; S spore; Scale bar: 10 µm. (b) Western Blot analysis on sporal proteins solubilized first in presence of 2.5% SDS (SDS extract) and then in a 100 mM DTT- containing solution (DTT extract). The antiserum reacted with a unique A. algerae sporal protein band at around 60 kDa in the DTT extract (arrow).