Table 2.
Mass spectrometry (LC–MS/MS) analysis of the 75-kDa and 180-kDa protein bands soluble in high concentration of 2-mercaptoethanol.
| Selected protein bands | Accession number or contig name | Putative function | Predicted molecular weight (kDa) | Matched peptides (number) |
|---|---|---|---|---|
| 75 kDa | WAQ68435 | PTP2 | 49 | 26* |
| KI0APB13YM20FM1 | Unknown | 29.3 | 5 | |
| KI0AEA11YJ13AHM1 | Elongation factor 1α | 52.9 | 4 | |
| WAQ68434 | PTP1 | 39 | 3 | |
| KI0ANB25YA19FM1 | Argonaute | 95 | 3 | |
| KI0AAA19YH14RM1 | Histone H3 | 14.8 | 2 | |
| KI0AEA15YN20FM1 | 20S proteasome subunit α | 25.6 | 2 | |
| KI0AGA12CC07FM1 | Actin | 41.1 | 2 | |
| 180 kDa | WAQ68437 | Putative PTP3 (PTP3b) | 121 | 43* |
| KI0AQA18YI18AHM1 | E3 ubiquitin ligase-like | 104.7 | 15 | |
| KI0APB13YM20FM1 | Unknown | 29.3 | 8 | |
| WAQ68435 | PTP2 | 49 | 8 | |
| WAQ68434 | PTP1 | 39 | 3 |
*The number of matched peptides was obtained from the incomplete protein sequences before identification of the complete sequence by 5’-RACE-PCR (PTP2) or RNAseq sequencing data assembly (putative PTP3). Both nucleic and proteic sequences are listed in the Table S3. kDa kiloDalton.