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. 2023 May 30;8:27. doi: 10.1038/s41536-023-00301-7

Fig. 1. Grafted human cerebral organoids recapitulate characteristics of the cerebral cortex in stroke mice.

Fig. 1

a Representative image showing the cerebral cortex of mice stained with GFAP on d 3 after stroke. The area outlined by the white dotted line indicates the infarct core. Scale bar, 200 μm. b Diagram showing human cerebral organoids transduced with LV-EF1a-EGFP and their transplantation, timeline of immunostaining. c Diagram showing the site of the organoids transplant. d Schematic showing corticogenesis of developing human cerebral cortex. e–h Images showing GFP+ grafts expressing VZ markers PAX6 and SOX2 and subplate marker TBR2 at 45 d after transplantation. i–m Images showing GFP+ grafts expressing deeper layers markers TBR1, FOXP2 and CTIP2 and upper layers markers SATB2 and BRN2 at 60 d after transplantation. Images showing GFP+ grafts expressing NeuN (n), CaMKII (o), STEM123 (p) GABA (q) and Ki67 (r) at 60 d after transplantation. Images showing GFP+ grafts expressing STEM121 (s) Syn (t) at 80 d after transplantation. Scale bars, e–n and r, 50 μm, o–q and s, 20 μm, t, 20 μm. HO Hoechst 33258 dye.