Fig. 4. LncKCND1 binds to YBX1 and increases the expression of YBX1.
A Representative western blot band of YBX1 protein pulled down by LncKCND1 (up). Lower: Extracted-ion chromatogram (XIC) for the YBX1 by MS (down). B Prediction of the overall interaction propensity between LncKCND1 and YBX1 by catRAPID. C RIP experiments were performed using YBX1 and negative IgG antibody. The purified RNA was used for further qRT-PCR assays (n = 3; **P < 0.01). D Representative western blot band of the YBX1 after transfection of LncKCND1 siRNA (n = 6, **P < 0.01 vs. si-NC). E Relative mRNA levels of the YBX1 after transfection of LncKCND1 siRNA (n = 5, **P < 0.01 vs. si-NC). F Representative western blot band of the YBX1 after overexpression of LncKCND1 under Ang II stimulation (n = 6, *P < 0.05, **P < 0.01). G Relative mRNA levels of the YBX1 after overexpression of LncKCND1 under Ang II stimulation (n = 5, *P < 0.05, **P < 0.01). H Representative western blot band of the YBX1 after overexpression of LncKCND1 under TAC surgery (n = 3, *P < 0.05).