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. 2023 May 30;14(5):344. doi: 10.1038/s41419-023-05852-7

Fig. 5. Overexpression of YBX1 attenuates cardiomyocytes hypertrophy.

Fig. 5

A The protein level of YBX1 in hearts of Sham and TAC mice (n = 4, **P < 0.01 vs. Sham). B Relative expression level YBX1 protein in primary cardiomyocytes induced by Ang II for 48 h (n = 6, *P < 0.05, vs. Control). C After CMs transfection with Vector or YBX1 for 6 h, Ang II was added for 48 h. CMs were stained by α-actinin and DAPI for nuclear staining (n = 4, *P < 0.05, **P < 0.01; scale bars, 20 μm). D, E Relative mRNA level of YBX1 and hypertrophy marker genes ANP, BNP and β-MHC in CMs after transfection of YBX1 (n = 5, *P < 0.05). F Representative western blot bands of the β-MHC and YBX1 after YBX1 transfection under Ang II stimulation (n = 6, *P < 0.05). G Effect of overexpression of YBX1 on CMs cellular ATP content under Ang II stimulation (n = 6, *P < 0.05). H Representative images of CMs’ JC-1 staining after YBX1 overexpression (n = 3, *P < 0.05, **P < 0.01; scale bars, 50 μm). I ROS level, determined in CMs transfected with YBX1, under Ang II stimulation (n = 3, *P < 0.05, **P < 0.01; scale bars, 50 μm). J Representative images of mitochondrial ROS staining after YBX1 overexpression under Ang II stimulation (n = 6, *P < 0.05, **P < 0.01; scale bars, 50 μm).