Skip to main content
. 2023 May 30;14(5):344. doi: 10.1038/s41419-023-05852-7

Fig. 6. LncKCND1 regulates cardiac hypertrophy by targeting YBX1.

Fig. 6

A After CMs transfection with si-NC or si-YBX1 for 6 h, CMs were transfected with Vector or LncKCND1 for 6 h, then Ang II was added for 48 h. CMs were stained by α-actinin and DAPI for nuclear staining (n = 4, *P < 0.05, **P < 0.01; scale bars, 20 μm). B Relative mRNA levels of ANP, BNP and β-MHC in CMs after co-transfection (n = 5, *P < 0.05). C Representative western blot bands of the β-MHC after co-transfection of LncKCND1 and YBX1 siRNA under Ang II stimulation (n = 6, *P < 0.05, **P < 0.01). D Effect of silencing YBX1 on CMs cellular ATP content promoted by overexpression of LncKCND1 under Ang II stimulation (n = 6, **P < 0.01). E Representative images of CMs’ JC-1 staining after co-transfection (n = 3, *P < 0.05, **P < 0.01; scale bars, 50 μm). F ROS level was determined in CMs transfected with LncKCND1 and YBX1 siRNA under Ang II stimulation (n = 3, **P < 0.01; scale bars, 50 μm). G Representative images of mitochondrial ROS staining after co-transfection (n = 4, **P < 0.01; scale bars, 50 μm).