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. 2023 May 30;13:8758. doi: 10.1038/s41598-023-35799-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Concentration and size distribution of paired milk EV and serum EV samples. NTA was used to determine (a) the adjusted nanoparticle concentration (particles/ml) and (b) the modal size (diameter) of n = 9 paired milk EV and serum EV samples. Donors 2, 4, 6, 8 and 9 were non-allergic donors and donors 1, 3, 5 and 7 were allergic donors. Bars represent mean ± SD from three captures of 60 s per sample. (c) NTA histograms show the non-adjusted concentration and the size distribution of paired milk EVs (green) and serum EVs (pink). (d) The median adjusted nanoparticle concentration and modal size of n = 9 paired milk EV samples and serum EV samples. Significance was tested by 2-tailed non-parametric Mann–Whitney T test, p < 0.001 (***).