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. 2023 May 30;13:8758. doi: 10.1038/s41598-023-35799-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Titration of APC detection signals is proportional to input EV numbers. (a) Flow cytometry dot plots of five input amounts of milk EVs (green) and serum EVs (pink) from donor 1 depicting the percentage of APC⁺ beads (relative to single beads) (For donors 2 and 3, see Supplementary Fig. S3). The bar graphs show the percentage of APC⁺ beads as mean ± SD of three donors (donor 1, donor 2, donor 3). (b) The floating bar graph depicts the median + min and max values (Donors 1–3) of MFI APC relative to CD9, CD63, CD81 capture beads at different EV doses. a.u. = arbitrary unit.