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. 2023 May 30;13:8758. doi: 10.1038/s41598-023-35799-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Protein distribution on milk EVs and serum EVs with pan-tetraspanin detection. (a) MFI APC signal of pan-tetraspanin detection associated to the 37 MACSPlex proteins in n = 9 paired milk EV (green) and serum EV (pink) samples (5 × 10⁸ EVs). In grey proteins with associated MFI APC signals not significantly higher than the MFI APC of the corresponding internal isotype control (non-detected). For statistical analysis see Supplementary Table S4 in which significance was determined by non-parametric Kruskal–Wallis Test with post-hoc Dunn’s multiple comparison. (b) Venn Diagram depicting unique and common proteins of milk EVs and serum EVs. a.u. = arbitrary unit.