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. 2023 May 30;13:8758. doi: 10.1038/s41598-023-35799-w

Figure 5.

Figure 5

Heterogeneous distribution of tetraspanins on EVs from milk and serum. MFI APC of CD9 (a), CD63 (b) and CD81 (c) capture bead populations (x-axis) detected with standard pan-tetraspanin antibody (pan-t: αCD9, αCD63 and αCD81) or single tetraspanin antibody (αCD9, αCD63 or αCD81) (y-axis). Bar graphs delimited by minimum and maximum value with median of MFI APC values from n = 9 EV samples per body fluid. Non-parametric Kruskal–Wallis Test with post-hoc Dunn’s multiple comparison was performed to compare pan-tetraspanin with each single tetraspanin detection, p ≥ 0.05 (ns), p < 0.05 (*), p < 0.01 (**), p < 0.001 (***). a.u. = arbitrary unit.