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. 2023 May 30;13:8758. doi: 10.1038/s41598-023-35799-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of pan-tetraspanin and single tetraspanin detection of milk EVs and serum EVs bound to different protein capture bead populations. MFI APC signals of capture bead populations (identified in Fig. 3) on milk EVs (a) and serum EVs (b) from pan-tetraspanin detection (pan-t: αCD9, αCD63 and αCD81) in comparison to each individual tetraspanin detection (αCD9, αCD63 or αCD81). Bar graphs delimited by minimum and maximum value with median of MFI APC values from n = 9 EV samples per body fluid. Non-parametric Kruskal–Wallis Test with post-hoc Dunn’s multiple comparison was performed to compare pan-tetraspanin with each single tetraspanin detection, p ≥ 0.05 (ns), p < 0.05 (*), p < 0.01 (**), p < 0.001 (***). a.u. = arbitrary unit.