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. 2023 May 30;9:20. doi: 10.1038/s41540-023-00284-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Relative contribution of medium components to cell culture at 168 h (regular mode). The top ten components and the sum of the remaining 19 components, i.e., Others, are indicated. Fivefold cross-validation of GBDT in the regular mode was applied. Standard deviations of five replicates are indicated. B Relative contribution of medium components to cell culture at 96 h (the time-saving mode). The top ten components and the sum of the remaining 19 components, i.e., Others, are indicated. Fivefold cross-validation of GBDT in the time-saving mode was applied. Standard deviations of five replicates are indicated. C Chemical component concentrations in the optimized media predicted by the regular and time-saving modes. The six chemical components, in which the concentrations were significantly different between the two modes, are shown. The green and blue circles represent the media optimized by the regular and time-saving modes. D Abundance of FBS in the optimized media. The concentrations of FBS in the optimized media predicted by the regular and time-saving modes are indicated as green and blue circles, respectively. The gray horizontal line indicates the serum concentration in the commercial EMEM medium.