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. 2023 May 17;14:1196372. doi: 10.3389/fendo.2023.1196372

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Copyright © 2023 Zhang, Pei, Wu, Xia, Wang, Huang, Li, Xie, Du and Lin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell experiment. (A) Survival analysis showed the effect of LGALS3 expression on prognosis. (B) The difference in LGALS3 expression between normal samples and tumor samples was found in the TCGA database. (C) Relative expression of LGALS3 in tumor and paracancerous tissues in LUAD and LGALS3 was highly expressed in tumor tissues compared with adjacent tissues (D) qRT-PCR to evaluate the level of LGALS3 expression 5 days after transfection and siRNA sequences could result in a significant decrease in LGALS3 expression (P < 0.001). (E, F) CCK8 assay showed that, after LGALS3 knockdown, the cells showed a significant reduction in viability. (G) EdU staining assay indicated that downregulation of LGALS3 expression repressed cell proliferation in LUAD cell lines. *P < 0.05, ***P < 0.001.