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. 2023 May 17;14:1159634. doi: 10.3389/fmicb.2023.1159634

Figure 4.

Figure 4

Tetmemena polymorpha n. sp., giant morph (A–G) specimens and resting cysts (H–K) from life. (A, B) Ventral (A) and dorsal (B) view of freely motile specimens, showing the body shape, the large adoral zone of membranelles, the very long paroral membrane, and the large food vacuoles (arrows). (C) Ventral view of the anterior portion of the body. (D) Ventral view of the posterior portion of body, showing the transverse cirri distinctly protruding from posterior body end. (E–G) Ventral views of slightly squashed cells, showing the nuclear apparatus and the food vacuoles containing small morph cells (arrows). (H–K) Optical sections (H–J) and surface view (K) of developing (H) and mature resting cysts (I–K), showing the thick wall (opposite arrowheads), the large spines (arrows), and the macronuclear nodules (asterisks). Note the macronuclear nodules fuse after cyst maturation. AZM, adoral zone of membranelles; BC, buccal cirri; CC, caudal cirri; FC, frontal cirri; FVC, frontoventral cirri; MA, macronuclear nodules; PM, paroral membrane; TC, transverse cirri. Scale bars 100 μm (A, B, E–G), 50 μm (C, H–K), and 25 μm (D).