Figure 5.

Expression of molecules involved in RAS signaling pathway in the lungs of F. tularensis LVS infected Hap-I and Hap-II mice. Adult and old Hap-I (hypertensive) and Hap-II (normotensive-control) TG mice (n = 4 per group/time) were infected intranasally with 1 × 10⁴ CFU of F. tularensis LVS. RNA isolated from lungs on day five post-infection was used for quantitation of expression of human (A1) and mouse (A2) At12 genes, and other indicated molecules involved in RAS signaling pathway by qRT-PCR (B1,B2,C1,C2). Relative quantification of transcripts (ΔRQ) was done by subtracting the change in transcript levels of each control subject post-F. tularensis LVS infection. The p-values were determined using two-way ANOVA. *p < 0.05, **p < 0.01; ***p < 0.001.