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. 2023 Mar 7;16(1):133–159. doi: 10.1016/j.jcmgh.2023.03.001

Figure 1.

Figure 1

FSP1 is overexpressed in human HCC. (A) Schematic representation of pathways involved in ferroptosis regulation. (B) Left: TCGA data revealed upregulation of FSP1 in 49 human HCC tissues compared with corresponding NT liver tissues. Right: Waterfall plot showed upregulation of FSP1 in 37% (18/49) of patients with HCC retrieved from TCGA by at least 2-fold. (C) Left: TCGA data revealed downregulation of TF in human HCC tissues compared with corresponding NT liver tissues. Right: TCGA data revealed GPX4 is not upregulated in human HCC tissues compared with corresponding NT liver tissues. (D) Left: RT-qPCR results from an expanded cohort of 70 human HCC tissues from our institute demonstrated upregulation of FSP1 compared to corresponding NT liver tissues. Right: Waterfall plot showed upregulation of FSP1 in 63% (44/70) of patients with HCC by at least 2-fold. (E) Analysis of TCGA data revealed patients with HCC with FSP1 upregulation (Z-score > 1) are associated with poorer overall and disease-free survival. (F) TCGA data revealed positive correlations between expression of FSP1 with expressions of known NRF2-targeted genes including TKT, ME1, MTHFD1L, NQO1, AKR1B10, and AKR1C1 in human HCC and NT liver tissues. ∗∗∗∗P < .0001 vs NT. B‒D, F: Student t test. E: Kaplan-Meier followed by log-rank test. RSEM = RNA-Seq expression estimation by expectation maximization.