Figure 10.

iFSP1 promoted anti-tumorigenic immune cells infiltration. (A) tSNE plot revealed clusters of major cell populations isolated from mouse HCC tumor treated with iFSP1 or vehicle. (B) tSNE plots showed the selected marker genes of major immune cell populations. (C) Percentage change of major immune cell clusters revealed the increase of DCs, macrophages, and T cells in tumor upon iFSP1 treatment. (D) tSNE plot illustrated further sub-clustering of selected myeloid cells. (E) Percentage change of myeloid cell subsets demonstrated iFSP1 treatment led to an increase in all DC subsets but differential effect on macrophages and monocytes. (F) Heatmap showing expression of major histocompatibility complex II genes in the myeloid subsets indicated that DCs expressed the highest level of antigen-presenting signature. (G) Volcano plot showing differentially expressed genes between Mac-1 and Mac-2 revealed that phagocytosis-related genes were enriched in Mac-2. P-value < .05 and log₂ (fold change) > 0.25. (H) Heatmap showing expression of phagocytosis-related genes in the myeloid subsets indicated that Mac-2 expressed the highest level of phagocytic signature. B: Expression: normalized expression. C, E: Error bars indicate 95% confidence interval. F, H: Expression: Z-score normalized mean expression.