Table 3.
Primer | Sequence |
---|---|
Human FSP1 (ChIP) | Forward: TTCAAAGTTCAAGGCCGTTC Reverse: TGGTCGTCTTCCCGAGTTAC |
Human FSP1 | Forward: GGAGACAGGGTTCGCCAAAA Reverse: CCCGTGGCCAGGATAAGATG |
Human NRF2 | Forward: GGTTGCCCACATTCCCAAAT Reverse: AGCAATGAAGACTGGGCTCT |
Human KEAP1 | Forward: CCTTCAGCTACACCCTGGAG Reverse: AACATGGCCTTGAAGACAGG |
Human MAF | Forward: CCCGTCCTCTCCCGAGTTTT Reverse: ACACTGGTAAGTACACGATGCT |
Human 18S | Forward: GAGGATGAGGTGGAACGTGT Reverse: AGAAGTGACGCAGCCCTCTA |
Mouse Afp | Forward: AAGCAAAAGCCTGAACTGACA Reverse: TCAACTTTGGACCCTCTTCTG |
Mouse Gapdh | Forward: GGGTGTGAACCACGAGAAAT Reverse: CCTTCCACAATGCCAAAGTT |
Afp, Alpha fetoprotein; ChIP, chromatin immunoprecipitation; FSP1, ferroptosis suppressor protein 1; KEAP1, kelch-like ECH-associated protein 1; MAF, MAF BZIP transcription factor; NRF2, nuclear factor erythroid 2-related factor 2.