Table 4.

shRNA Target Sequences and Insert Sequences for Luciferase Reporter Assay

shRNA	Target sequence
shNRF2-55	GCTCCTACTGTGATGTGAAAT
shNRF2-94	AGTTTGGGAGGAGCTATTATCC
shKEAP1-57	GCGAATGATCACAGCAATGAA
shKEAP1-76	GTGGCGAATGATCACAGCAAT
shFSP1-23	CGGGCAAGTTTAATGAGGTTT
shFSP1-24	CATTTCTTACTCGGTGACTTT
shMAF-42	TCAGTGGGATACGCCACATTT
shMAF-58	TGGAAGACTACTACTGGATGA
Insert	Sequence
WT	GGGGTCGGGGACCAGCACGAGTGCTGAGTCACGCCCCGCCCGGG
MUT	GGGGTCGGGGACCAGCACGAGTAAAAAAAAACGCCCCGCCCGGG

FSP1, ferroptosis suppressor protein 1; KEAP1, kelch-like ECH-associated protein 1; MAF, MAF BZIP transcription factor; MUT, mutated; NRF2, nuclear factor erythroid 2-related factor 2; shRNA, short hairpin RNA; WT, wild-type.