Figure 2.

Relative CCT activity of protein body fractions in relation to fl2 gene dosage. CCT activity is expressed as fold induction over activity in normal endosperm. A, Protein body fractions (mean normal value was 0.237 nmol min⁻¹ mg⁻¹ protein). B, ER fractions. Proteins were extracted from kernels harvested 18 DAP with the triploid endosperm genotypes +/+/+ (0 doses), +/+/fl2 (one dose), +/fl2/fl2 (two doses), and fl2/fl2/fl2 (three doses) and were fractionated by centrifugation through Suc gradients. Bars represent means of three separate experiments ± se