Figure 5.

Immunoblot analysis of proteins from soybean cells treated with Tm. Proteins were extracted from whole cells as described in “Materials and Methods” and equal protein loading was confirmed by staining of duplicate gels with Coomassie Brilliant Blue (data not shown). A, Immunoblot probed with anti-BiP antibody. The arrowhead indicates BiP cross-reacting band of about 75 kD. B, Immunoblot probed with anti-PDI antibody. The arrowhead indicates cross-reacting PDI doublet. The slight shift in mobility of PDI in Tm-treated cells represents the inhibition of glycosylation. C, Immunoblot probed with anti-PI4K antibody. The arrowhead indicates a 65-kD cross-reacting band.