Table 1.
Frequency of chromosomal abnormalities induced by caffeine and Pb(NO3)2 in Lens culinaris Medik.
| Conc.of mutagens (%) and (ppm) | Total No. of PMCs Obser. |
Prophase-I |
Metaphase-I/II |
Anaphase-I/II |
Telophase-I/II |
Total No. of Abn. PMCs Obser. |
Total % of Abn. PMCs Obser. |
|||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Univalents | Multivalents | % of Abn. PMCs | Univalents | Multivalents | Precocious Mov | Stray chromosomes | Stickiness | % of Abn. PMCs | Laggards | Bridges | Unequal Sep. | % of Abn. PMCs | Laggards | Bridges | Unequal Sep. | Micro nucleate | Multi nucleate | Disturbed Polarity poleritl |
Cytomixis | % of Abn. PMCs | ||||
| Control | 275 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Caffeine (%) | ||||||||||||||||||||||||
| 0.10 | 236 | – | 1 | 0.42 | – | 1 | – | 1 | 1 | 1.27 | 1 | – | – | 0.42 | 1 | – | – | 1 | 1 | – | – | 1.27 | 8 | 3.38 |
| 0.25 | 252 | 1 | 1 | 0.79 | – | 1 | – | 1 | 2 | 1.58 | 1 | – | 1 | 0.79 | 1 | – | – | 1 | 1 | 1 | – | 1.58 | 12 | 4.74 |
| 0.50 | 263 | 2 | 2 | 1.52 | 1 | – | 2 | 1 | 2 | 2.28 | 2 | 1 | – | 0.82 | 2 | 1 | 1 | 1 | 2 | 2 | 1 | 3.80 | 23 | 8.42 |
| 0.75 | 275 | 2 | 3 | 1.81 | 2 | 2 | 2 | 3 | 4 | 4.72 | 2 | 1 | 2 | 1.81 | 3 | 2 | 2 | 3 | 3 | 3 | 2 | 6.54 | 41 | 14.88 |
| 1.0 | 285 | 3 | 3 | 2.10 | 1 | 3 | 2 | 5 | 4 | 5.26 | 3 | 2 | 2 | 2.45 | 3 | 2 | 2 | 4 | 5 | 3 | 3 | 7.71 | 50 | 17.52 |
| Pb(NO3)2 (ppm) | ||||||||||||||||||||||||
| 5 | 247 | 1 | 1 | 0.76 | – | 1 | 1 | 1 | 1 | 1.61 | 1 | – | 1 | 0.80 | 1 | – | – | 1 | 1 | 1 | – | 1.61 | 12 | 4.78 |
| 10 | 258 | 1 | 2 | 1.20 | 1 | – | 1 | 2 | 2 | 2.32 | 2 | – | 1 | 1.16 | 2 | 1 | 1 | 2 | 2 | 1 | 1 | 3.87 | 22 | 8.55 |
| 15 | 262 | 1 | 3 | 1.92 | 1 | 2 | 2 | 3 | 3 | 4.19 | 2 | 1 | 1 | 1.52 | 3 | 1 | 2 | 3 | 3 | 2 | 2 | 6.10 | 35 | 13.73 |
| 20 | 270 | 2 | 3 | 2.29 | 2 | 3 | 2 | 4 | 4 | 5.55 | 2 | 1 | 2 | 1.85 | 3 | 2 | 3 | 3 | 4 | 4 | 2 | 7.77 |
46 | 17.46 |
| 25 | 278 | 3 | 3 | 2.57 | 3 | 4 | 5 | 5 | 6 | 8.27 | 4 | 2 | 4 | 3.59 | 4 | 2 | 3 | 4 | 4 | 4 | 3 | 8.63 1111 |
63 | 23.06 |