Figure 10.

Mitochondrial respiration is affected by extracellular vesicles derived from the lungs of mice exposed to brand B tobacco flavor. C57BL/6J male and female mice were exposed air and tobacco-flavored e-cig aerosols 2 h/day for three consecutive days in SCIREQ whole-body exposure chamber. Mice were euthanized ∼24 h postexposure and exosomes were isolated from the lung tissue. MLE15 cells were grown in Seahorse plates and treated with isolated exosomes. Twenty-four hours later, Sea horse mitostress assay was run and oxygen consumption rate (OCR) extracellular acidification rate (ECAR) parameters were acquired, and normalized by total cells. A, MLE cells treated with air group extracellular vesicles (EVs) and B, MLE cells treated with tobacco flavor exposed EVs. t-test, ***p < .001 versus control, N = 3.