Skip to main content
. 2023 Apr 13;193(2):146–165. doi: 10.1093/toxsci/kfad033

Figure 2.

Figure 2.

Differential cell counts in bronchoalveolar lavage fluid by exposure to brand A, PG/VG, menthol, and tobacco flavors with and without nicotine in C57BL/6J and BALB/cJ mice. C57BL/6J and BALB/cJ male and female mice were exposed to PG/VG, menthol, menthol 6 mg nicotine, tobacco, and tobacco 6 mg nicotine 2 h/day for three consecutive days in SCIREQ whole-body exposure chamber. Mice were euthanized ∼24 h postexposure and the BALF was collected. A and B, Total cell counts were performed by staining with AO/PI dye. C and D, Macrophages (F4/80+), E and F, neutrophils (Ly6B.2), G and H, Th lymphocytes (CD4+), and I and J, Tc lymphocytes (CD8+) were assessed as percentages of CD45+ parent cell population by flow cytometry. Data are shown as mean ± SEM. *p < .05, **p < .01, and ***p < .001 versus respective air group per strain (2-way ANOVA) (N = 4–8 per group).