Figure 6.

ENDS flavors induced acute lung injury-associated biomarkers. C57BL/6J male and female mice were exposed to PG/VG, menthol, menthol 6 mg nicotine, tobacco, and tobacco 6 mg nicotine 2 h/day for three consecutive days in SCIREQ whole-body exposure chamber. Mice were euthanized ∼24 h postexposure, and lung tissues were homogenized for immunoblotting by SDS-PAGE. A, TRPA-1; B, NLRP3; C, SP-D; D, PAI-1 protein abundance were measured and normalized to GAPDH and β-actin loading controls. Data shown for respective bands and the densitometry values were plotted as mean ± SEM. *p < .05, **p < .01, and ***p < .001 versus respective air group per strain (2-way ANOVA) (N = 3–4 per group).