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. 2023 Apr 13;193(2):146–165. doi: 10.1093/toxsci/kfad033

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Figure 9. — A, Inflammatory and Metabolic gene expression alterations by acute exposure to PG/VG, menthol, menthol with nicotine, tobacco, tobacco with nicotine aerosols. C57BL/6J male and female mice were exposed to three days (2 h/day) air, PG/VG, menthol, menthol + nicotine, tobacco, and tobacco + nicotine aerosols. RNA isolated from lungs were hybridized with Nanostring codesets and the inflammatory and metabolic gene alterations were determined. Genes that ±1.5-fold change altered significantly compared to the Air group were compared. p < .05, 1-way ANOVA, N = 6 per group. B, Protein alterations by acute exposure to PG/VG, menthol, menthol with nicotine, tobacco, tobacco with nicotine aerosols. C57BL/6J male and female mice were exposed to three days (2h/day) air, PG/VG, menthol, menthol + nicotine, tobacco, and tobacco flavor + nicotine aerosols. Protein changes were determined by proteomics analysis and presented in a Venn diagram. Proteins significantly altered by 1.5-fold are listed (p < .05 vs air group), N = 3 per group.