Table I.
Strain | Mutation | Doubling Time | Chlorophyll in Trimers | Fd-Mediated NADP+ Photoreduction Ratea |
---|---|---|---|---|
h | % | μmol NADPH/nmol PSI/h | ||
WT | None | 40 | 46 | 1.44 |
D10 | R111C | 38 | 72 | 0.81 |
DE | K117A | 42 | 67 | 1.48 |
DF | K131A/K135A | 58 | 41 | 1.30 |
DΔCt | Δ105–140 | 36 | 65 | 0.91 |
Cell growth was followed by optical density measurements of the cell suspension at 730 nm.
Rates of NADP+ photoreduction were measured at a Fd concentration of 0.05 μm.