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The primer sequences used for gene sequencing and qPCR experiments.
| Primer | Sequence | ||
|---|---|---|---|
| Bacteria | V3-V4 | Forward | 5’ CCTACGGGNGGCWGCAG 3’ |
| Reverse | 5’ GACTACHVGGGTATCTAATCC 3’ | ||
| 518F | Forward | 5’ CCAGCAGCCGCGGTAATACG 3’ | |
| 800R | Reverse | 5’ TACCAGGGTATCTAATCC 3’ | |
| Fungi | ITS3-ITS4 | Forward | 5’ TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGCATCGATGAAGAACGCAGC 3’ |
| Reverse | 5’ GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTCCTCCGCTTATTGATATGC 3’ | ||
