Figure 5:
ERG regulates TH9 function in the absence of PU.1 and ETV5 in chronic AAD model. (A) Schematic of AAD model using transgenic mice of specified genotype. (B) Total number of cells in the lung. (C) Number of cells per ml in the BAL fluid. Number of CD4 T cells (D), B cells (E), neutrophils (F), eosinophils (G), macrophages (H), and CD8 T cell counts (I) in the lung. (J-O) Frequency of IL-9, IL-13, IL-5, IL-4, IL-10 and IFN-γ producing CD4 T cells in the lung. (P) IL-9 secretion from lung CD4 T cells co-cultured with naïve CD11c+ cells in the presence of IL-33 and A.f. (100mg/ml) for 72 hours ex vivo. Data are mean of 5 mice per experiment and representative of two independent experiments. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.