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. 2023 Feb 28;30(2):183–192. doi: 10.2174/0929866530666221202123011

Figure 4.

Figure 4

The effect of GHa4R on biofilms of S. aureus. The antibiofilm activity of (A) GHa and (B) GHa4R during biofilm formation. The biofilm eradication effect of (C) GHa and (D) GHa4R. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. nontreated controls. (E-L) The antibiofilm activity of GHa4R was observed by using a fluorescence microscope. (E–H) GHa4R inhibited the biofilm formation of S. aureus. The bacteria were treated with (E) 0, (F) 1.6, (G) 3.1, and (H) 6.2 μM GHa4R. (I-L) GHa4R eradicated the 24 h-biofilm of S. aureus. The 24 h-biofilms were exposed to (I) 0, (J) 25, (K) 50, and (L) 100 μM GHa4R. The biofilms were stained with SYTO and colored green. The black area means the biofilms were absent or destroyed.