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. 2023 May 22;11(5):e006230. doi: 10.1136/jitc-2022-006230

Figure 5.

Figure 5

FUBP1 promoted M2-like macrophage polarization through c-Myc. (A) Expression of c-Myc in M0, M1 and M2-like macrophages derived from THP-1 cells was measured using western-blot assay. (B) Expression of FUBP1 and c-Myc in M2-like macrophages derived from PBMC was tested by qPCR. (C–D) The expression of c-Myc was affected by FUBP1 at both mRNA and protein levels. (E–F) M2-c-Myclow cells and M2-c-Mychigh cells were established by transfecting c-Myc siRNA or c-Myc-plasmid, respectively. (G–H) Expression of M2-related markers in M2-like macrophages with NR_109 knockdown and overexpression were measured by western-blot and qPCR assays. (I) Migration of tumor cells cocultured with M2-c-Myclow or M2-c-Mychigh cells was analyzed. (J) Expression of c-Myc in M2-NR_109low cells and M2-NR_109high cells was detected using western-blot. (K) Knockdown NR_109 reversed the c-Myc-mediated high expression of M2-related markers and M. the migration of tumor cells in the coculture system. (N) Overexpression of FUBP1 could partially restore the expression of c-Myc in NR_109low cells. The statistical data are from three independent experiments and the bar indicates the SD values (*p < 0.05, **p < 0.01). FUBP1, far upstream element-binding protein 1.