Table 1.
Cytochrome P450s with relevance to insecticide response in Colorado potato beetles. Differentially expressed cytochrome P450s identified in L. decemlineata either exposed to different compounds or displaying resistance to select compounds. Methods used to quantify changes in cytochrome P450s are also provided. Abbreviations: Control (C), Resistant (R), Susceptible (S), Treated (T). Symbols: ↓ indicates down-regulation and ↑ indicates up-regulation.
| Cytochrome P450s | Insecticides | Conditions | Methods | References |
|---|---|---|---|---|
| 1 CYP4C1↓, CYP4G57↑, CYP6BJ1↑, CYP6BK17↓, CYP6BQ15↑, CYP6EF1↑, CYP6K1↑, CYP9Z12v1↑ | Imidacloprid | R vs. S adults | RNA-seq | [34] |
| CYP4C1↑, CYP4G15↑, CYP6A13↑, CYP9E2↑ CYP4G15↑ CYP6A23↑ |
Clothianidin Imidacloprid Spinosad |
T vs. C adults | qRT-PCR | [36] |
| CYP9Z14v2↑ | Fluazinam | T vs. C larvae | qRT-PCR | [38] |
| CYP6K1↑ | Imidacloprid | R vs. S adults | qRT-PCR | [39] |
| CYP6A13↓, CYP6A23↑, CYP6D4↓, CYP12A5↓ | Spinosad | T vs. C adults | qRT-PCR | [40] |
| CYP6BJa/b↑, CYP6BJ1v1↑, CYP9Z25↑, CYP9Z29↑ | Imidacloprid | T vs. C cells | qRT-PCR | [41] |
| CYP4BN13v1↑, CYP4BN15↑, CYP6BH2↑, CYP6BJ1↑, CYP6BQ17↑, CYP6BU1↓, CYP6EG1↑, CYP6EH1↑, CYP6EJ1↑, CYP9Z10↓, CYP12H2↑, CYP12J1↓ | Cyhalotrin | T vs. C larvae | qRT-PCR | [42] |
| 2 CYP6EH1↑, CYP6EZ1↑, CYP6FA1↑, CYP9A↑, CYP9Ya↑, CYP9Yb↑, CYP9Yc↑, CYP9Z14v1↑, CYP9Z25↑, CYP9Z26↑, CYP9Z28↑, CYP9Z29↑, CYP9Zd↑, CYP9Zi↑, CYP9Zj↑, CYP9Zk↑, CYP4BN13v4↑, CYP4Qb↑, CYP4c↑, CYP4e↑ | Imidacloprid | R vs. S adults | qRT-PCR | [43] |
| CYP6K1↑ CYP4D2↑, CYP6K1↑, CYP49A1↑ |
Chlorothalonil Imidacloprid |
T vs. C adults | RNA-seq | [44] |
1 Presented are targets that showed absolute log2 fold-changes higher than 5.0. 2 Shown are transcripts that displayed increased transcript levels greater than 5.0-fold in resistant versus susceptible insects.