Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 12;97(5):e00438-23. doi: 10.1128/jvi.00438-23

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Itakura et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PMC Copyright notice

FIG 3 — RABV M interacts with TSG101 via the L-domain. (A) Schematic representation of RABV M and the L-domain mutants. (B) Colocalization of RABV M and TSG101. SK-N-SH cells stably expressing TSG101-Venus were infected with RABV, immunostained with anti-RABV M at 24 hpi, and analyzed using confocal microscopy. Arrowheads show colocalization. Scale bar, 50 μm. (C to F) Coimmunoprecipitation of RABV M with TSG101. HA- or FLAG-tagged RABV M and TSG101 were coexpressed in 293T cells and coimmunoprecipitated using anti-HA magnetic beads. Immunoblotting was performed with anti-HA or -FLAG antibody. Bar graphs show the relative precipitation efficacy (IP/input) of FLAG compared with that of HA from a representative experiment following quantification via ImageJ.