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. 2023 May 11;97(5):e00337-23. doi: 10.1128/jvi.00337-23

FIG 5.

FIG 5

The regulatory effects of NS1 and NS2 on viral RNA transcription and replication are conserved in influenza A and B viruses. The effects of NS1 and/or NS2 on viral RNA transcription and replication in RNP/NA reconstitution systems derived from H1N1 (A/WSN/1933 [H1N1/WSN] and A/California/04/2009 [H1N1/CA04]) and H3N2 (A/Hong Kong/01/1968 [H3N2/HK68]) subtypes of influenza A virus (A) and B/Yamagata/16/1988 (IBV/Yam) of influenza B virus (B) in HEK 293T cells were determined. The steady-state levels of RNAs and proteins were determined by primer extension analysis (top) and Western blotting (bottom) at 24 hpt. The graphs show the relative mean intensities of viral RNA normalized to 5S rRNA. The data represent the means ± SD from three biological replicates. Asterisks represent a significant difference from the control group (by one-way ANOVA with Dunnett’s post hoc test) (*, P < 0.05; **, P < 0.01; ***, P < 0.001).