TABLE 2.

Previously characterized ZIKV amino acid substitutions and their phenotypes^a

Protein	Position	Residue	Phenotype(s)
Capsid	106	Ala	Enhanced infectivity and fitness in mosquitoes and IFNAR KO mice (32, 35)
prM	123	Val	Enhanced infectivity in mosquito cells; increased frequency of fetal demise in IFNAR KO mice (36)
prM	139	Asn	Enhanced infectivity in human and murine neural progenitor cells; increased microcephaly severity in BALB/c mice (37)
Envelope	443	Asn	Enhanced infectivity and virulence in IFNAR KO mice and mosquitoes; reduced infectivity and entry into mosquito cells; reduced neurovirulence in BALB/c mice (83, 84, 115, 116)
NS1	982	Val	Increased NS1 antigenemia and infectivity in mosquitoes; enhanced ability to block IFN induction (31, 85)

^aColumns 1 to 3 indicate the viral protein, position relative to the viral polyprotein, and amino acid residue, respectively. Column 4 describes the phenotype associated with the specific amino acid residue as previously demonstrated.