TABLE 3.
Quantification of relevant immunoblot protein abundancea
| Figure | Protein | Mean abundance ± SEM at 24 hpib | Mean abundance ± SEM at 48 hpib | P value (24 hpi) | P value (48 hpi) |
|---|---|---|---|---|---|
| Fig. 3A | pIRF3 S386 | 0.347 ± 0.004 (ZIKV/Dakar) 0.294 ± 0.035 (ZIKV/Malaysia) |
0.812 ± 0.137 (ZIKV/Dakar) 1.128 ± 0.159 (ZIKV/Malaysia) |
0.2639 | 0.2080 |
| Fig. 3A | pIRF3 S396 | 0.378 ± 0.103 (ZIKV/Dakar) 0.282 ± 0.071 (ZIKV/Malaysia) |
0.985 ± 0.387 (ZIKV/Dakar) 1.033 ± 0.458 (ZIKV/Malaysia) |
0.4926 | 0.9407 |
| Fig. 3A | IκB | 0.236 ± 0.060 (ZIKV/Dakar) 0.176 ± 0.053 (ZIKV/Malaysia) |
0.231 ± 0.084 (ZIKV/Dakar) 0.088 ± 0.037 (ZIKV/Malaysia) |
0.4991 | 0.2268 |
| Fig. 4A | pSTAT1 | 0.805 ± 0.011 (ZIKV/Dakar) 0.764 ± 0.080 (ZIKV/Malaysia) |
1.517 ± 0.147 (ZIKV/Dakar) 0.366 ± 0.128 (ZIKV/Malaysia) |
0.6562 | 0.0044 |
| Fig. 4A | STAT1 | 0.588 ± 0.286 (ZIKV/Dakar) 0.393 ± 0.195 (ZIKV/Malaysia) |
1.460 ± 0.810 (ZIKV/Dakar) 0.358 ± 0.174 (ZIKV/Malaysia) |
0.6060 | 0.3052 |
| Fig. 4A | STAT2 | 0.260 ± 0.052 (ZIKV/Dakar) 0.094 ± 0.030 (ZIKV/Malaysia) |
0.490 ± 0.089 (ZIKV/Dakar) 0.037 ± 0.017 (ZIKV/Malaysia) |
0.0649 | 0.0328 |
| Fig. 4A | RIG-I | 0.659 ± 0.128 (ZIKV/Dakar) 0.602 ± 0.203 (ZIKV/Malaysia) |
1.607 ± 0.555 (ZIKV/Dakar) 0.640 ± 0.210 (ZIKV/Malaysia) |
0.8238 | 0.2171 |
| Fig. 4A | IFITM1 | 0.016 ± 0.008 (ZIKV/Dakar) 0.007 ± 0.001 (ZIKV/Malaysia) |
0.613 ± 0.099 (ZIKV/Dakar) 0.024 ± 0.009 (ZIKV/Malaysia) |
0.2845 | 0.0262 |
| Fig. 4A | OAS1 | 0.190 ± 0.128 (ZIKV/Dakar) 0.088 ± 0.045 (ZIKV/Malaysia) |
0.294 ± 0.192 (ZIKV/Dakar) 0.024 ± 0.019 (ZIKV/Malaysia) |
0.5193 | 0.2935 |
| Fig. 4A | Mx1 | 0.073 ± 0.045 (ZIKV/Dakar) 0.044 ± 0.034 (ZIKV/Malaysia) |
0.835 ± 0.274 (ZIKV/Dakar) 0.042 ± 0.023 (ZIKV/Malaysia) |
0.4866 | 0.1008 |
| Fig. 4A | IFIT1 | 0.380 ± 0.079 (ZIKV/Dakar) 0.180 ± 0.063 (ZIKV/Malaysia) |
1.223 ± 0.263 (ZIKV/Dakar) 0.228 ± 0.058 (ZIKV/Malaysia) |
0.1219 | 0.0575 |
a
The mean abundance of relevant proteins at 24 and 48 hpi from three independent experiments were quantified by densitometry and are depicted. The corresponding P-values are presented in columns 5 and 6.
b
Mean protein abundance derived from three independent experiments (n = 3). Statistical analysis was performed with a two-tailed unpaired t test with Welch’s correction at each time point.