TABLE 4.
Primer sequences for cloning and qRT-PCRa
| Primerb | Sequence (5′–3′) | Source |
|---|---|---|
| Primers for cloning | ||
| pEF N-FLAG vector F | AAACCCGCTGATCAGCCT | This paper |
| pEF N-FLAG vector R | GCTGAATTCCTTGTCATCGTCATCC | This paper |
| ZIKV NS3 F | GACAAGGAATTCAGCGAGGGAGAGTTCAAGCTTAGGACG | This paper |
| ZIKV NS3 R | CTGATCAGCGGGTTTGATAGGCCAGCCAAACAGGAAGA | This paper |
| pcDNA3.1+ ZIKV/Dakar and ZIKV/Malaysia NS5 vector F | GTTTAAACCCGCTGATCAGCCT | This paper |
| pcDNA3.1+ ZIKV/Dakar and ZIKV/Malaysia NS5 vector R | CAGCTTGGGTCTCCCTATAGTGAG | This paper |
| pcDNA3.1+ ZIKV/Dakar NS5 and ZIKV/Malaysia NS5 F | GGGAGACCCAAGCTGATGGGAGGTGGAACGGGAGAG | This paper |
| pcDNA3.1+ ZIKV/Dakar NS5 R | TCAGCGGGTTTAAACTTACAGCACTCCAGGTGTGGAC | This paper |
| pcDNA3.1+ ZIKV/Malaysia NS5 R | TCAGCGGGTTTAAACTTATAACACTCCAGGTGTGGACCC | This paper |
| Primers for TaqMan qRT-PCR | ||
| ZIKV NS3 F | GAGGGAGAGTTCAAGCT | This paper |
| ZIKV NS3 R | GATAGGCCAGCCAAAC | This paper |
| ZIKV NS3 probe (with quencher) | 56-FAM/TTCATGAGTTCCACAAAGGTCTTCCTTTGC/3IABkFQ | This paper |
| Primers for SYBR green qRT-PCR | ||
| IFNB | NA | PPH00384F; Qiagen |
| TNF | NA | PPH00341F; Qiagen |
| IL-6 | NA | PPH00560C-200; Qiagen |
| MX1 | NA | PPH01325A; Qiagen |
| IFITM1 | NA | PPH05981C; Qiagen |
| DDX58 | NA | PPH20774A-200; Qiagen |
| OAS1 | NA | PPH01324A-200; Qiagen |
| RPL13A F | GCCCTACGACAAGAAAAAGCG | 27 |
| RPL13A R | TACTTCCAGCCAACCTCGTGA | 27 |
a
All sequences used for SYBR green qRT-PCR are of human origin.
b
F, forward; R, reverse; NA, not available.