FIG 1.

The dynamic changes in HDAC6 expression during PDCoV infection. (A) LLC-PK1 cells were infected with PDCoV at an increasing multiplicity of infection (MOI = 0.1, 0.5, or 1). At 12 hpi, cells were collected for Western blotting using antibodies against HDAC6, α-tubulin, Ac-α-tubulin, and PDCoV N protein. (B) LLC-PK1 cells were infected with PDCoV (MOI = 0.5). At 6, 12, 18, and 24 hpi, cells were collected for Western blotting assay to detect the expression of HDAC6, α-tubulin, Ac-α-tubulin, PDCoV N, and β-actin.