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. 2023 May 3;97(5):e00054-23. doi: 10.1128/jvi.00054-23

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FIG 1 — Screen of new compounds that block PRRSV GP2a/GP4-CD163 interaction. (A) Scheme diagram for the BiFC assay constructs between CD163-SRCR5 or PRRSV envelope proteins GP2a/GP4 and the fragments of Venus protein VN155(I152L) or VC155, respectively. (B) BiFC screening images for select positive compounds that inhibit the PPI between SRCR5-VN and GP2a-VC proteins. NC, DMSO Ctrl. Bar = 250 μm. (C) Relative fluorescence intensity of the SRCR5/GP2a BiFC screening results for all 20 positive compounds. Mean ± SD; n = 3. (D) Comparison for the relative fluorescence intensity of the SRCR5/GP2a and SRCR5/GP4 BiFC screening results for the 20 positive compounds. Mean ± SD; n = 3. (E) BiFC screening images for select positive compounds that inhibit the PPI between SRCR5-VN and GP4-VC proteins. NC, DMSO Ctrl. Bar = 250 μm. (F) Chemical structure of the 5 identified strong positive compounds by both BiFC assay.