FIG 1.

p27 is present in the F protein of infectious sucrose-purified RSV (spRSV) A and B. Equal amounts of spRSV were resolved by reducing SDS-PAGE gel and analyzed by Western blotting. A representative blot of duplicates is shown. Membranes were probed with palivizumab (anti-site II), RSV7.10 (anti-p27), or D25 (anti-site Ø) monoclonal antibodies. F0, uncleaved F protein (~70 kDa); F1+p27, partially cleaved p27 remained on the N terminus of F1 subunit (~60 kDa); F1, fully cleaved subunit (~50 kDa).