FIG 3.

Progressions of infection and F protein cleavage are different between HEp-2 and A549 cell lines infected with RSV/A or RSV/B. Percentage of double-positive RSV-infected HEp-2 (A and C) or A549 (B and D) cells per day postinoculation is shown. Cells were infected with RSV/A/Bernett, RSV/A/ON, RSV/A/Tracy, RSV/B/18537, or RSV/B/BA at an MOI of 0.07. Cells were costained with fluorescence-conjugated MAb (palivizumab [anti-site II], RSV7.10 [anti-p27], or D25 [anti-site Ø]). Antigenic site II is surrogate measure of total F protein, while antigenic site Ø is pre-F specific.