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S-fragment deletions allow trans-complementation of cis-acting replication components. (A) Schematic of the trans-complementation experiment which involved co-transfecting BHK-21 cells with mCherry replicons containing S-fragment deletions together with a WT ptGFP, ptGFP-3B3T>K or ptGFP-3DGNN replicon. Fluorescent protein expression was monitored hourly for 24 h. The data show (B) ptGFP positive cells per well or (C) mCherry positive cells per well at 8 h posttransfection (n = 2 ± SD).
