Predicted Foxc1 binding site is required for embryonic enhancer activity. (A) Schematic of GFP reporter construct with Bmp6 intronic enhancer with intact, scrambled, and deleted sequences for the predicted Foxc1 binding site upstream of the hsp70l promoter driving eGFP. (B) GFP reporter expression pattern driven by the Bmp6 intronic enhancer with intact, scrambled, or deleted sequence for the Foxc1 binding site in 7 dpf (stage 23, (Swarup, 1958)) stickleback embryos. Strong expression was seen in the distal edge of the developing pectoral fin (arrowhead) and median fin (arrow) for the enhancer with an intact sequence of the predicted Foxc1 binding site (left). Severely reduced GFP expression in all domains of enhancer activity was detected for enhancers with the scrambled (middle) or deleted (right) sequences of the predicted Foxc1 binding site. Scale bar = 100 μm.