Figure 1.

MED17 promotes root development in Arabidopsis. A, Root phenotype of Col-0 and med17 seedlings. Length of the scale bar in the image is 5 mm. B, Graph showing the length of primary roots in Col-0 and med17 seedlings. C, Graph showing the number of lateral roots in Col-0 and med17 seedlings. D, Graph showing the number of lateral root primordia (LRPs) in different stages of development in Col-0 and med17 seedlings. In (B–D), Col-0 and med17 seedlings were grown on ½ MS for 9 days. Lengths of primary roots were measured using ImageJ. Counting of LRP was done according to Malamy and Benfey (1997). Data shown are average of three independent biological replicates of at least 25 seedlings. Bar plots represent mean values, and error bars denote Se. Statistical difference was assessed by P-value as assessed by one-way ANOVA and Tukey's HSD post hoc test. E, Root tip stained with PI. Lines are showing size of the meristem. Length of the scale bar in the image is 100 µm. F, Graph showing size of root meristems in Col-0 and med17 seedlings. G, Graph showing cortical cell numbers of Col-0 and med17 seedlings. H, Root tip stained with EDU. Length of the scale bar in the image is 50 µm. In (E–H), seedlings were grown on ½ MS for 4 days and processed for confocal imaging. ImageJ was used to measure meristem length and count the cortical cell number. Data shown are average of three independent biological replicates containing at least 10 seedlings. Bar plots represent mean values, and error bars denote Se. Statistical difference has been depicted by P-value as assessed by one-way ANOVA and Tukey's HSD post hoc test. For all the graphs, P-value of 0.05 or lower (P ≤ 0.05) was considered statistically significant, whereas P-value greater than 0.05 (P > 0.05) was considered non-significant (ns).